Translational_Unit

Part:BBa_K3477007:Design

Designed by: Mathilda Johansson   Group: iGEM20_Chalmers-Gothenburg   (2020-10-20)


Gene coding for his-tagged PEG-DH enzyme including BBa_0034 RBS, Bba_J23106 promoter and Bba_0010 te


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1784
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
    Illegal PstI site found at 659
    Illegal PstI site found at 795
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal NheI site found at 1419
    Illegal SpeI site found at 1785
    Illegal PstI site found at 659
    Illegal PstI site found at 795
    Illegal PstI site found at 1799
    Illegal NotI site found at 7
    Illegal NotI site found at 1792
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal BamHI site found at 896
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1785
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 56
    Illegal PstI site found at 659
    Illegal PstI site found at 795
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 56
    Illegal SpeI site found at 1785
    Illegal PstI site found at 659
    Illegal PstI site found at 795
    Illegal PstI site found at 1799
    Illegal AgeI site found at 1403
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

The genetic sequence comes from Sphingomonas macrogoltabidus and was found on Uniprot (https://www.uniprot.org/uniprot/A9ZM00)


References